By Henry Beadnell
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Extra resources for A guide to typography,: In two parts, literary and practical; or, The reader's handbook and the compositor's vade-mecum
If cDNA is synthesized on the basis of total RNA, even the use of a T-primer does not prevent the synthesis of a large excess of background cDNA on ribosomal RNA as a template. On the basis of SSP, a method was developed that involves T4 DNA ligase-mediated ligation of a suppression adapter to double-stranded cDNA, followed by PCR with a T-primer and a primer corresponding to the external part of the adapter (Figure 2A) (Lukyanov et al. 1997). Such PCR is accompanied by selective amplification of the cDNA fraction comprising the T-primer structure.
45 References . . . . . . . . . . . . . . . . . . . . . . . . . 48 1. ) are under the control of specific regulatory genes. To understand the underlying molecular mechanisms, the genes involved in their regulation should be revealed and studied. At present, the majority of methods of molecular biology involved in unraveling such problems are based on polymerase chain reaction (PCR), which has made it possible to work with small amounts of biological material. However, the use of PCR requires information on the sequence of the DNA under study.
Taken together, these approaches allow one to analyze complex DNA samples, from searching sequences of interest to determining complete structures of the respective genes. Keywords: Selective PCR suppression, PCR suppression, suppression of the PCR, PS, pan handle, suppression adapter, inverted repeats, annealing temperature, primer concentration, suppression sequence, preparation of full-size cDNA, small amount of biological material, selective amplification, differential display, ordered differential display, targeted differential display, shortcoming, disadvantage, normalized cDNA libraries, evolutionary conserved sequences, search for promoter sites, chromosome walking, in vitro cloning, multiplex PCR.