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Extra resources for Bending of a Curved Tube of Circular Cross Section

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In the case of blood samples, simple boiling releases enough DNA for successful amplification. 5-ml thin-walled reaction tubes or, as indicated, in 96-well microtiter plates. It comprises reaction buffer (optimized for magnesium chloride), deoxynucleotide triphospates (dNTPs), the oligonucleotide primers, a thermostable DNA polymerase (usually Taq DNA polymerase), which synthesizes DNA by incorporating dNTPs and extending the annealed primers, and, finally, the template to be amplified. 2. Steps Involved in a PCR Cycle The initial step of PCR involves incubation of around 94°C for 5 min to denature the target genomic DNA into single strands.

Maule J (2000) Pulsed-field gel electrophoresis. In: Rapley R (ed) The nucleic acid protocols handbook. Humana, Totowa, NJ 6. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503 7. Alwine JC, Kemp DJ, Stark GR (1977) Method for the detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74:5350 8. Aquino de Muro M, Rapley R (2002) Gene probes: principles and protocols.

J Mol Biol 98:503 7. Alwine JC, Kemp DJ, Stark GR (1977) Method for the detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl paper and hybridization with DNA probes. Proc Natl Acad Sci USA 74:5350 8. Aquino de Muro M, Rapley R (2002) Gene probes: principles and protocols. Humana, Totowa, NJ 9. Zamore PD, Tuschi T, Sharp PA, Bartel DP (2000) RNAi: double-stranded RNA directs the ATP-dependent cleavage of mRMA at 21 to 23 nucleotide intervals. Cell 101 (1):25–33 10. Nykanen A, Haley B, Zamore PD (2001) ATP requirements and small interfering RNA structure in the RNA interference pathway.

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