
By Osheroff N., Bjornsti M.A.
DNA Topoisomerase Protocols is an remarkable selection of state of the art experimental protocols for investigating DNA constitution, topology, and DNA topoisomerase functionality. defined by means of professional experimentalists who've perfected the innovations, those time-proven equipment make the most of new methods to check adjustments in DNA topology (linking quantity, knotting, catenation, and rest) and DNA constitution (bending), in addition to to evaluate chromosome constitution via FLP-mediated recombination and to investigate bacterial nucleoid constitution. state of the art protocols additionally element the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from quite a few assets, together with baculovirus, bacterial, yeast, and mammalian expression structures.
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